Concepts and techniques in genomics and proteomics by Nachimuthu Saraswathy

By Nachimuthu Saraswathy

Advent to genes and genomes; The human genome undertaking; Genomes of version organisms; excessive ability vectors; DNA sequencing tools; Genome mapping; Genome sequencing tools; Genome series meeting and annotation; practical genomics; advent to proteomics; Two-dimensional gel electrophoresis of proteins; Mass spectrometry for proteomics; Protein id by way of Peptide Mass Fingerprinting (PMF); Protein sequencing concepts; Phosphoproteomics; Glycoproteomics

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Coli genome and it is only 1/30 of the human genome. It is the first animal genome sequenced; the complete sequence has been available since 1998. Clone-by-clone strategy was Genomes of model organisms 37 followed for full-length genome sequencing. Two important centres involved in C. elegans genome sequencing are the Washington University Genome Sequencing Center (WUGSC) supported by the National Human Genome Research Institute (NHGRI), USA, and the Sanger Institute, supported by the Wellcome Trust, UK.

It is a multicellular filamentous fungi having medicinal, industrial and agricultural importance. Neurospora was known to scientists when it was used to prove the one-gene, one-enzyme hypothesis by Beadle and Tantum in 1946. Being a multicellular fungus, it was explored for cellular differentiation and the sexual cycle. It is a good model to study epigenetic mechanisms. Repeat Induced Point (RIP) mutation is one such phenomenon by which a particular genome protects itself from mobile genetic elements.

5 The Human Genome Organization (HUGO) The HGP is an international effort, and scientists from more than 18 countries are involved in this project. To ensure the efficient functioning of this multinational, multi-phased project, coordination was important. A separate agency called the Human Genome Organization (HUGO) was established in 1989. The main objective of agency was to coordinate the sequencing project in order to avoid unnecessary competition among scientists, to avoid duplication of work and also to encourage the exchange of scientific material and data relevant to genome sequencing among scientists.

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