Alkaliphiles: Genetic Properties and Applications of Enzymes by Koki Horikoshi

By Koki Horikoshi

The pioneering paintings of Dr. Koki Horikoshi at the discovery, learn and functions of alkaliphilic micro organism are defined right here of their entirety. The isolation, distribution and taxonomy of alkaliphilic microorganisms in addition to their cellphone constitution and body structure are mentioned for a simple figuring out of those entities. The molecular biology and genome sequencing of a few alkaliphilic bacterial traces also are presented.

Part II of the quantity makes a speciality of enzymes of alkaliphiles and their purposes. those comprise alkaline proteases, starch-degrading enzymes and various others. a few of these enzymes are at the moment in vast advertisement use as laundry detergent ingredients and in wastewater treatment.

Alkaliphiles are a comparatively fresh box of analysis that might without doubt supply avenues to quite a lot of extra discoveries and functions for a brand new iteration of employees in bioscience and technology.

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Additional resources for Alkaliphiles: Genetic Properties and Applications of Enzymes

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Halodurans C-125 and its many mutants have been made by conventional mutation methods. Recently, whole genome sequence was determined and annotated (Takami et al. 2000). 1 pH Values of Culture Media A. External pH values Alkalinity in nature may be the result of the geology and climate of the area, of industrial processes, or promoted by biological activities. The most stable alkaline environments on earth are the soda lakes and soda deserts distributed throughout the world in tropical and sub-tropical areas.

It was therefore concluded that all of the peptidoglycans of the alkaliphilic Bacillus strains so far examined are of the Aly-type of peptidoglycan, which is found in the majority of the strains of the genus Bacillus. C. Acidic polymers in the cell wall of alkaliphilic B. halodurans C-125 Most strains of group 2 can grow at neutral pH and require the presence of sodium ions. The same acidic amino acids and uronic acids are found in much smaller quantities in the walls prepared from bacteria grown at neutral pH.

2). 1. 0 (Fig. 4). 5 M. Other cations such as K^, NH4^, Rb^, Cs^ and Ca^ ^ did not show this stimulating effect. 2 Germination oi' Bacillus sp. No. 2 M. ''Germination is expressed as the optical densin- reduced (%) after 60 min at 37°C. 7). (Reproduced from T. Kudo and K. Horikoshi, Agtic. Biol. , 47, 666 (1983)) 50 y-^ r 40 1 30 - 20 - 10 - 0 1 \ \ / \ / \ IZ 1 1 1 8 9 10 11 \— 12 pH Fig. 4 Effect of pH on germination of spores of Bacillus sp. No. 2b-2. (Reproduced from T. Kudo and K. Horikoshi, Agric.

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